April 6-7, 2017 | Lincoln, Nebraska
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2017 NRIC speakers
portrait of Schmitz

 

Dr. Bob Schmitz
University of Georgia
Transgene mediated epimutagenesis leads to heritable phenotypic variation
11:20 - 11:50am, Thursday 6 April 2017
ABSTRACT:
There exists extensive natural epigenetic and cryptic variation in plant genomes, some of which is linked to heritable phenotypic variation. Accessing this source of variation to improve plant traits is cumbersome as it often relies on the identification and incorporation of rare events into breeding programs. We describe a novel methodology termed “epimutagenesis” to rapidly generate phenotypic variation by randomly demethylating the genome of Arabidopsis thaliana via transgenic expression of a DNA demethylase. Not only does this system lead to widespread loss of DNA methylation, it also leads to the production of heritable phenotypic variation. Application of epimutagenesis to other plant species will lead to expression of alleles typically silenced by DNA methylation, uncovering previously hidden genetic variation.
BIO:
Bob Schmitz earned his B.Sc. in Molecular & Cellular Biology from the University of Arizona and his Ph.D. in Genetics from the University of Wisconsin-Madison. His postdoctoral training was performed at the Salk Institute. In 2013, Bob joined the Genetics Department at the University of Georgia where his lab is studying how phenotypic plasticity and diversity are driven by natural epigenetic variation. His lab uses a combination of molecular genetics and epigenomic approaches on plant populations to understand the impact that natural epigenetic alleles (epialleles) have on life history traits.